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LdBOB Promastigote Media
| Reagent | Final concentration | Amount |
|---|---|---|
| M199 media | - | 1 sachet |
| FBS | 10% | 100 mL |
| Sodium bicarbonate | 1 g/L | 1 g |
| Glutamax (100× stock) | 1× | 10 mL |
| Folic acid | 4.4 mg/L | 4.4 mg |
| Adenosine | 27 mg/L | 27 mg |
| 2.5 mg/mL Hemin | 10 mg/L | 4 mL |
| ddH2O | - | 900 mL |
| Total | - | 1000 mL |
