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Binding Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| HEPES pH 7.5 (1 M) | 20 mM | 200 μL |
| KCl (1 M) | 10 mM | 100 μL |
| CaCl2 (1 M) | 1 mM | 10 μL |
| MnCl2 (1 M) | 1 mM | 10 μL |
| BSA (30%) ∗ | 0.1% | 33.33 μL |
| ddH2O | n/a | 9.65 mL |
| Total | n/a | 10 mL |
Can be stored at 4°C for 1 weeks.
Recommended: Adding BSA here as well as in Wash Buffer and Digitonin-300 Wash Buffer reduces beads sticking to tube walls without reduction in binding to cells/nuclei.
