Wash Buffer

Reagent	Final concentration	Amount
HEPES pH 7.5 (1 M)	20 mM	1000 μL
NaCl (5 M)	150 mM	1500 μL
Spermidine (2 M)	0.83 mM	12.5 μL
BSA (30%)	0.1%	166.7 μL
Roche Complete Protease Inhibitor EDTA-Free tablets	n/a	1 tablet
ddH2O	n/a	47.5mL
Total	n/a	50 mL

Can be stored at 4°C for 1 weeks.