Digitonin-300 Wash Buffer

Reagent	Final concentration	Amount
HEPES pH 7.5 (1 M)	20 mM	1000 μL
NaCl (5 M)	300 mM	3000 μL
Spermidine (2 M)	0.83 mM	12.5 μL
Digitonin (5%)	0.1 μg/mL	100 μL
BSA (30%)	0.1%	166.7 μL
Roche Complete Protease Inhibitor EDTA-Free tablets	n/a	1 tablet
ddH2O	n/a	46 mL
Total	n/a	Close to 50 mL

Can be stored at 4°C for 1 weeks.