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Tagmentation Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| MgCl2 (1 M) | 10 mM | 150 μL |
| Digitonin-300 Wash buffer | n/a | 14.8 mL |
| Spike-inAmpR∗ (56.97 amol/L stock solution) | 0.1899 amol/L | 50 μL |
| Total | n/a | 15 mL |
Always prepare fresh.
Spike-inAmpR used here is at a very low concentration (56.97 amol/L), this concentration can be achieved by sequential dilutions of the original purified PCR product Spike-inAmpR. The addition of the Spike-inAmpR is optional, and can be replaced by H20.
