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CUT&Tag DNA resuspension Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl pH=8.0 (1 M) | 10 mM | 20 μL |
| EDTA (500 mM) | 0.1 mM | 2 μL |
| RNAse A∗ | 1/1200 in volume | 1.7 μL |
| Nuclease-free dH2O | n/a | 1976.3 μL |
| Total | n/a | 2 mL |
Always prepare fresh.
RNAse A purchased from Sigma-Aldrich (R4642) is at a concentration of 20–40 mg/mL in solution form (50% glycerol, 10 mM Tris-HCL pH 8.0). And this is the recommended form of RNase A by us.
