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PCR Reaction Components
| Component | 25 μL reaction |
|---|---|
| Spike-inAmpR | 2 μL |
| H2O | 8.5 μL |
| i5 primer (10 μM) | 1 μL |
| i7 primer (10 μM) | 1 μL |
| PowerUp™ SYBR™ Green Master Mix (2×) | 12.5 μL |
In this 25 μL qPCR system, the concentration of the Spike-inAmpR fragment is known: 65.6 nM × (2/25) = 5.25 nM. This is the standard qPCR reaction.
