PCR Reaction Components
| Component | 50 μL reaction |
|---|---|
| CUT&Tagged genomic DNA | 20 μL |
| ddH2O | 1 μL |
| i5 primer (10 μM) | 2 μL |
| i7 primer (10 μM) | 2 μL |
| NEBNext High-Fidelity 2× PCR Master Mix | 25 μL |
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