pA-Tn5 Dialysis Buffer

Reagent	Final concentration	Amount
HEPES-KOH pH 7.2 (1 M)	100 mM	160 mL
NaCl (5 M)	200 mM	64 mL
Glycerol (100%)	20%	320 mL
Triton X-100 (100%)	0.2%	3.2 mL
EDTA pH 7.2 (0.5 M)	0.2 mM	640 μL
DTT (1 M)	2mM	3.2mL
ddH2O	n/a	1048.96 mL
Total	n/a	1600 mL

Filter sterilize, store at 4°C (should be made fresh prior to dialysis and pre-chilled).