Dispase II 20× stock Solution

Reagent	Final concentration	Amount
HEPES pH 7.4 (500 mM)	50 mM	2.25 mL
NaCl (5 M)	150 mM	675 μL
Dispase® II (neutral protease, gradeII)	40 U/mL	900 U (1 g)
ddH2O	n/a	Make up the volume to 22.5 mL
Total	n/a	22.5 mL

Make 500 μL aliquots in microcentrifuge tubes, and store at −20°C until use