Differentiation medium for Pax6⁺ granule cells

Reagent	Final concentration	Amount
Neurobasal medium without phenol red	N/A	46.5 mL
KSR	5%	2.5 mL
Glutamax (100 ×, 200 mM)	2 mM	500 μL
2-ME (50 mM)	0.1 mM	100 μL
Wnt3a (100 ng/μL)	20 ng/mL	10 μL
BMP4 (10 μM)	0.5 nM	2.5 μL
Pen/Strep (100×)	1×	500 μL
Total	N/A	50 mL