Differentiation medium for Calbindin⁺ Purkinje cells

Reagent	Final concentration	Amount
Neurobasal medium without phenol red	N/A	48 mL
B27-Plus	1×	1 mL
Glutamax (100 ×, 200 mM)	2 mM	500 μL
L-Ascorbic acid (200 mM)	0.2 mM	50 μL
Wnt3a (100 ng/μL)	20 ng/mL	10 μL
BMP4 (10 μM)	0.5 nM	2.5 μL
Pen/Strep (100×)	1×	500 μL
Total	N/A	50 mL