REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-Calbindin	ImmunoStar (1:500)	Cat# 24427
Mouse anti-Pax6	Synaptic Systems (1:300)	Cat# 153011
Goat anti-Rabbit IgG (H+L), Alexa FluorTM488	Invitrogen (1:500)	A-11008
Goat anti-Mouse IgG (H+L), Alexa FluorTM647	Invitrogen (1:500)	A-21235
Chemicals, peptides, and recombinant proteins
DAPI	Sigma	Cat# D9564
Tamoxifen	Sigma	Cat#T5648
Mounting Medium	Vector Laboratories	Cat#H-1000
Poly-L-ornithine solution	Sigma	P4957
Poly-L-ornithine hydrobromide	Sigma	P3655
Laminin	Sigma	L2020
L15 medium	Gibco	11415064
0.05% Trypsin/EDTA	Gibco	25300-054
Fetal Bovine Serum (FBS)	Gibco	10270106
Bovine serum albumin (BSA)	Sigma	A9647
DNAse I	Serlabo	LS002138
Neurobasal medium without phenol red	Gibco	12348-017
B27 supplement	Gibco	17504-044
L-glutamax (100×)	Gibco	35050-061
Mouse epidermal growth factor (EGF)	Thermo Fisher	PMG8041
Mouse basic fibroblast growth factor (bFGF)	Thermo Fisher	PMG0035
HEPES	Gibco	12509079
Insulin	Sigma	I0516
KnockOut ™ Serum Replacement (KSR)	Gibco	10828010
2-Mercaptoethanol (50 mM) (2-ME)	Gibco	31350010
Pen/Strep (100×)	Gibco	15140-122
BMP4	R&D	5020-BP-010
Recombinant mouse Wnt-3a protein	R&D	1324-WNP-010
Neurobasal-Plus	Gibco	A3582901
B27-Plus	Gibco	A3582801
L-Ascorbic acid	Sigma	A4403
Experimental models:Organisms/strains
Sox2CreERT2	The Jackson Laboratory	JAX stock #017593
Gt(ROSA)26SortdTom	The Jackson Laboratory	JAX stock #007914
Oligonucleotides
Forward primer for Cre: 5′-CCAATTT ACTGACCGTACACCAA-3′	IDT	N/A
Reverse primer for Cre: 5′-CTGATCC TGGCAATTTCGGCTA-3′	IDT	N/A
Forward primer for tdTomato: WT-5′-AAGG GAGCTGCAGTGGAGTA-3’; Mut-5′-CTGTTCCTGTACGGCATGG-3′	IDT	N/A
Reverse primer for tdTomato: WT-5′-CCGAA AATCTGTGGGAAGTC-3’; Mut-5′-GGCATTAAAGCAGCGTATCC-3′	IDT	N/A
Software and algorithms
ZEISS ZEN Blue software	Zeiss	https://www.zeiss.com/
ImageJ	ImageJ	https://imagej.nih.gov/ij/download.html
FV-OSR software	Olympus	https://www.olympus-lifescience.com/en/
Other
Videomicroscope	Zeiss	AxioObserver 7
Confocal microscope	Olympus	FV-1200
0.2 μm Syringe filter	Sartorius	16532-K