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. 2021 Aug 24;4:1001. doi: 10.1038/s42003-021-02526-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Components of the microfludic set up. Air and CO₂ from the wall lines (WL) are mixed in airflow mixer (AFM) and passed through air filter (AF) before reaching the pump (OB1-MK3). Flow path 1 (FP1) with air splitter (ASpl) shows eight outlets connected to the reservoirs with specific drug concentrations. Time and flow rate of the reservoir content are regulated by the distributor (MUX) and piezo pump (OB1-MK3). The FP1 continues to a bubble trapper (BT) and a digital flow sensor (DFS) into 2 chained Ibidi chips (ICh 1,2) and finaly to the waste bottle (WBot). Flow path 2 (FP2) for mock treated spheroids is represented by single reservoir and the two chained Ibidi chips (ICh 3,4). A feedback loop (FL) connects each digital sensor (DFS) to the OB1-MK3 pump to maintain desired flow rate. b Photograph of the Ibidi Luer chip with 8 Matrigel encapsulated spheroids, and brighfield microscopic image of SW620 spheroid after 7 days of continuous media flow. Scalebar = 100 µm.