Fig. 3.

Pgf-DE-Ki mice, a fully functional Pgf-KO model achieved by knocking in the Pgf-DE variant unable to bind and activate VEGFR1, show robust reduction of CNV and protection from vascular leakage. A. CNV volumes measured 7 days after laser-induced damage by Isolectin B4 staining of RPE-choroid flat mounts. B. Q ualitative fundus fluorescein angiography in C57BL6/J, Pgf-KO, and Pgf-DE-Knock-in mice acquired at three different times (early – 1 min, intermediate – 5 min, late – 15 min) after intraperitoneal delivery of fluorescein at Days 3, 7, and 14 after laser-induced damage. Reproduced under Creative Common CC-BY license (Apicella et al., 2018).