Table 2.
Summary of the ante-mortem tuberculosis (TB) diagnostic tests and post-mortem analysis in the goats under study.
| Herd | Animals | SIT testa | CIT testb | IGRAc | P22 ELISA (serum)d | P22 ELISA (oral fluid)d | TB lesions in lungs | TB lesions in head and pulmonary lymph nodes |
|---|---|---|---|---|---|---|---|---|
| M. bovis -infected | 64 | 55 (85.9%) | 37 (57.8%) | 38 (59.4%) | 56 (87.5%) | 22 (34.4%) | 54 (84.4%) | 60 (93.7%) |
| TB-free | 113 | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) |
SIT, Single intradermal tuberculin test: protocol and interpretation was performed according to Council Directive 64/432/EEC and Royal Decree RD2611/1996.
CIT, Comparative intradermal tuberculin test: protocol and interpretation was performed according to Council Directive 64/432/EEC and Royal Decree RD2611/1996.
IGRA, interferon-gamma release assay: an animal was considered positive to IGRA if the optical density (OD) of a sample stimulated with bovine PPD minus the OD of the aliquot stimulated with PBS (nil) was >0.05 and greater than the OD of the sample stimulated with avian PPD.
P22 ELISA, an animal was considered positive to ELISA p22 when the E% value was >150 (serum) or 100 (oral fluid).