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. 2021 Aug 11;8:689318. doi: 10.3389/fcvm.2021.689318

Figure 5.

Figure 5

NADPH oxidase (NOX4) mediates the negative regulatory effects of CCN1 on VE-cadherin expression. (A,C,E,G,I) Western blot analysis and (B,D,F,H,J) densitometry quantification of protein expression levels of VE-cadherin, NOX4, and CCN1 in (A,B) retina tissue of control mice and streptozotocin (STZ)-induced hyperglycemic mice on days 15 and 30 after modeling; (C,D) human retinal vascular endothelial cells (HRVECs) treated with serial dosages (50, 100, or 200 mM) of palmitic acid (PA) for 24 h; (E,F) HRVECs transfected with CCN1 plasmids or vector; (G,H) HRVECs transfected with siCCN1 or the negative control (siNC), followed by treatment with 100 mM PA or BSA for 24 h; (I,J) CCN1-KO HRVECs via CRISPR. *p < 0.05, **p < 0.01.