REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
1C1m anti-hu/muTEM1 scFv	Fierle et al.42	N/A
7G22 anti-huTEM1 scFv	This paper	N/A
sc78 scFv	Zhao et al.37	N/A
MORAb-004 scFv	US07807382B2	N/A
FMC63 anti-CD19 scFv	US7446179	N/A
TriloBiTE anti-hCD3 core	This paper	N/A
Rat anti-mouse CD31	Biolegend	RRID: AB_312908
Rabbit anti-mouse NG2	Merck Millipore	RRID: AB_11213678
Alexa Fluor 555-conjugated goat anti-human IgG (H+L)	Life Technologies	RRID:AB_2535854
Alexa Fluor 488-conjugated donkey anti-rat IgG (H+L)	Life Technologies	RRID:AB_2535794
Alexa Fluor 647-conjugated donkey anti-rabbit IgG (H+L)	Life Technologies	RRID:AB_25361833
Alexa Fluor 647-conjugated goat anti-human IgG, F(ab)2 fragment specific	Jackson Immunoresearch	RRID:AB_2337888
Alexa Fluor 647-conjugated goat anti-human IgG, Fc fragment-specific	Jackson Immunoresearch	RRID:AB_2337889
APC-conjugated mouse anti-human CD8 (clone SK1)	Biolegend	RRID:AB_2075388
BV785-conjugated mouse anti-human CD4 (clone OKT4)	Biolegend	RRID:AB_2561365
Alexa Fluor 700-conjugated mouse anti-human CD69 (clone FN50)	Biolegend	RRID:AB_493775
PE-conjugated mouse anti-human CD25 (clone BC96)	Biolegend	RRID:AB_314276
BV605-conjugated mouse anti-human PD1 (clone EH12.2H7)	Biolegend	RRID:AB_11124107
FITC-conjugated mouse anti-human CD3 (clone OKT3)	Biolegend	RRID:AB_571906
BV421-conjugated mouse anti-human Perforin (clone B-D48)	Biolegend	RRID:AB_11149688
PE-conjugated mouse anti-human Granzyme B (clone GB11)	BD Biosciences	RRID:AB_10561690
APC-conjugated Streptavidin	Biolegend	CAT#405207
HRP-conjugated mouse anti-human IgG, F(ab)2-fragment specific	Jackson Immunoresearch	RRID:AB_2339089
PE-conjugated goat anti-mouse IgG (H+L)	ThermoFisher Scientific	RRID:AB_2534043
Biological samples
Buffy coats	Service de Transfusion, Epalinges, Switzerland	N/A
Chemicals, peptides, and recombinant proteins
Recombinant human IL-2	Peprotech	CAT#200-02-50UG
Recombinant human IL-7	Miltenyi Biotec	CAT#130-095-367
Recombinant human IL-15	Miltenyi Biotec	CAT#130-095-765
hTEM1(Δn)-SpyC	Fierle et al. (2019)	N/A
h/mTEM1 ECD (biotinylated)	Fierle et al. (2019)	N/A
CD19 (biotinylated)	BioCat	CAT# CD9-H82E9
Critical commercial kits and assays
Caspase 3/7 apoptosis assay	Life Technologies	CAT#C10427
Human CD8 T cell isolation kit	Miltenyi Biotec	CAT#130-096-495
Human pan-T cell isolation kit	Miltenyi Biotec	CAT#130-096-535
CD3/CD28 T cell activator kit	Life Technologies	CAT#11161D
CytoTox 96 kit (LDH assay)	Promega	CAT#G1780
Human IFN-γ ELISA MAX Standard kit	Biolegend	CAT#430102
Human IL-2 ELISA MAX Standard kit	Biolegend	CAT#431801
Cytometric bead array, MultiCyt Qbeads customized	Bucher Biotec	CAT#90603
FAM-MGB-TaqMan probe (hCD248; Hs00535586_s1)	ThermoFisher Sci	CAT#4331182
FAM-MGB-TaqMan probe (hGAPDH; Hs02786624_g1)	ThermoFisher Sci	CAT#4331182
Experimental models: Cell lines
A673	ATCC	RRID:CVCL_0080
A673-KO	Synthego	https://www.synthego.com
2H11	ATCC	RRID:CVCL_6762
MS1	ATCC	RRID:CVCL_6502
SK-N-AS	ATCC	RRID:CVCL_1700
HEK293T	ATCC	RRID:CVCL_0063
AsPC-1	ATCC	RRID:CVCL_0152
Raji	ATCC	RRID:CVCL_0511
Jurkat-NFAT-Lucia	Invivogen	RRID:CVCL_KS47
Jurkat-NFAT-mCherry	Melita Irving, UNIL	N/A
Lewis lung carcinoma	ATCC	RRID:CVCL_4358
MC38	ATCC	RRID:CVCL_0A68
HEK293-6E	NRC Canada	RRID:CVCL_HF20
Experimental models: Organisms/strains
NOD/SCID/IL-2Rγ KO (NSG) mice	In-house colony	RRID:IMSR_NM-NSG-001
C57BL/6	In-house colony	RRID:IMSR_JAX:000664
Software and algorithms
GraphPad Prism (9.0)	GraphPad	https://www.graphpad.com/scientific-software/prism/
FlowJo v10	BD Lifesciences	https://www.flowjo.com/
Incucyte Base Software	Essen Bioscience	https://www.essenbioscience.com/en/products/software/incucyte-base-software/
Adobe Illustrator (CC)	Adobe	https://www.adobe.com/products/illustrator.html
Microsoft Excel (2019 MSO)	Microsoft	Product ID: 00414-50000-00000-AA651
Other
FectoPRO transfection reagent	Polyplus	CAT#116-010
Turbofect transfection reagent	Life Technologies	CAT#R0532
Cytotox Red reagent	Essen Bioscience	CAT#4632
QUANTI-Luc luciferase substrate	Invivogen	CAT#rep-qlc-1