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. 2021 Aug 17;2(8):100371. doi: 10.1016/j.xcrm.2021.100371

Figure 1.

Figure 1

Pancreatic enteroviral RNA in AAb-positive and T1D patients within both the endocrine and exocrine pancreas

(A–H) Detection and quantification of viral RNA (red), insulin (green), and DAPI (blue) in FFPE pancreases from control donors without diabetes (n = 14), donors without diabetes but expressing T1D-associated autoantibodies (AAb+) (n = 10), and donors with T1D (n = 15).

(A–C) Data are presented as (A) mean number of all viral RNA+ cells throughout the whole pancreas section, (B) the mean number of full-grade (full; ≥10 single puncta/cell) or low-grade (low; 1–9 single puncta/cell) infected cells, and (C) their localization in the pancreas within insulin-containing islets (I), within the periphery of 3 cells of insulin-containing islets (Peri), and within the exocrine pancreas (Ex).

(D and E) All (D) viral mRNA+ cells were normalized to the pancreas area of the respective section and (E) viral mRNA+ cells in the exocrine area were normalized to the exocrine area of the respective section.

(F and G) Viral mRNA+ β cells (F) co-staining with insulin (absolute value) and viral mRNA+ cells (G) within islets normalized to islet area (insulin+ stained area in mm2).

(H) Representative microscopical pictures from immunostainings of control, AAb+, and T1D pancreatic sections. Stainings were performed in two technical replicas. Scale bars depict 10 μm. ∗p<0.05 to control.