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. 2021 Aug 17;2(8):100371. doi: 10.1016/j.xcrm.2021.100371

Figure 3.

Figure 3

Enteroviral RNA is increased in the spleen in T1D

(A and B) Detection and quantification of viral RNA (red), CD45 in bright field (dark red), and DAPI in blue in FFPE spleen sections from autopsy from a subset of the analyzed pancreas donors without diabetes (n = 8), with T1D-associated autoantibodies (AAb+) (n = 8), and with T1D (n = 7) and presented as (A) mean number of viral RNA+ cells/spleen section area and (B) a correlation of the mean number of viral RNA+ cells/spleen section area with the mean number of viral RNA+ cells/pancreas section area.

(C and D) Representative microscopical pictures from immunostainings of control, AAb+, and T1D pancreatic sections of viral RNA/CD45 double staining (C) and viral RNA/DAPI double staining (D). White arrows show viral RNA+/CD45+ co-positive cells and black arrows viral RNA+/CD45− cells next to CD45+ cells. Stainings were performed in two technical replicas. Scale bars depict 10 μm. ∗p<0.05 to control.