REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-human TCR α/β FITC	BioLegend	#306706
anti-human CD3 PC7	Beckman Coulter	#737657
anti-human CD8 PE	Invitrogen	#MHCD0804
anti-murine TCR β-chain APC/Fire750	BioLegend	#109246
Streptavidin-BV421	BioLegend	#405225
Bacterial and virus strains
mCMV-ie2-ANLV	61	N/A
Chemicals, peptides, and recombinant proteins
Alt-R S.p. HiFi Cas9 Nuclease V3	IDT DNA	https://eu.idtdna.com
Alt-R CRISPR-Cas9 crRNA	IDT DNA	https://eu.idtdna.com
Alt-R CRISPR-Cas9 tracrRNA	IDT DNA	https://eu.idtdna.com
Alt-R Cas9 Electroporation Enhancer	IDT DNA	https://eu.idtdna.com
P3 Primary Cell 24 cuvettes Nucleofector™ Kit	Lonza	V4XP-3024
Critical commercial assays
Targeted locus amplification	Cergentis	https://www.cergentis.com
Chromium next GEM Single Cell VDJ V1.1.	10X Genomics	Rev D
Deposited data
scRNA-seq	This paper	https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE179566
Experimental models: cell lines
TPRKO	52	N/A
Experimental models: organisms/strains
NOD.Cg-PrkdcscidIl2rgtm1WjlTg(HLA-A/H2-D/B2M)1Dvs/SzJ (NSG/HHD)	56	JAX stock #014570
Oligonucleotides
See Table S2	This paper	N/A
Recombinant DNA
See Table S1	This paper	N/A
pMP71	Addgene	#108214
pCDH-EF1	Addgene	#72266
Software and algorithms
FlowJo v10	FlowJo, LLC	https://www.flowjo.com/
GraphPad Prism	Graphpad	https://www.graphpad.com/
SCANPY	62	https://scanpy.readthedocs.io/en/stable/
Cell Ranger (V3.0.2)	10X Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation
Other
Script for analysis of scRNA-seq data	This paper	https://github.com/SebastianJarosch/2021_Mueller_scRNA-OTR-vs-Tx