Figure 2.

Lipin1 deficiency results in a loss of PI3P close to Rab7 structures in myoblasts

(A) The confocal images show a representative 2xFYVE-GFP staining from 1 of 6 controls and patients. Dot plots (50 images/dot) show the means ± SDs number of 2xFYVE-GFP dots per patient versus control myoblast (Mann-Whitney U test).

(B) As in (A), but using immortalized myoblasts from 1 healthy donor treated with sh-Sc or sh-LPIN1. The plots (30–45 cells/condition) show the number of 2xFYVE-GFP dots (Mann-Whitney U test).

(C) Typical distribution of PI3P within the myoblast vesicles from 1 of 3 controls and patients. Box and whisker plots (50 images/condition) depict the percentage of proximity between PI3P and the given marker for 1 representative control and patient (Mann-Whitney U test).

(D) Myoblasts from 3 controls and 3 patients were stained for PI35P2 and late endosomal or lysosomal markers. Proximity was calculated as in (C) (unpaired t test). Results are from 1 representative of at least 3 independent experiments.

Scale bars, 10 μm.

See also Figure S1.