FIGURE 1.

CBD is antiproliferative and cytotoxic to glioma cell lines. (A) U87MG, U373MG, J3TBG, and SDT3G Glioma cells were treated with CBD isolate (0–20 μg/ml) for 96 h. Cell proliferation and dose response were measured via IncuCyte™ Red Object Count. Data are from n = 4 independent repeated experiments. Individual points expressed as mean ± SD, curve fit through a non-linear, variable slope regression (B) IC₅₀ values were determined from the curve fits via GraphPad Prism for both CBD isolate and extract at 96 h. Data are from n = 4 independently repeated experiments (C) U87MG, U373, J3TBG, and SDT3G Glioma cells were treated with CBD isolate or extract (0–20 μg/ml) for 96 h. Cytotoxicity was determined using the green fluorescent dye, YOYO-1. Data are from n = 4 independently repeated experiments, expressed as mean ± SD (D) SDT3G glioma cells treated with 0 μg/ml CBD after 48 h (i), SDT3G Glioma cells featuring formation of intracellular vesicles after 48 h of treatment with 7.5 μg/ml CBD isolate (ii) At 48 h, cell death is not occurring at this concentration. Red fluorescence represents nuclei of viable cells. Images were captured through a 10x Nikon objective.