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. 2021 Aug 11;12:698249. doi: 10.3389/fimmu.2021.698249

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Copyright © 2021 Feng, Min, Liang, Chen, Chen, Zhou, Deng, Liu and Hou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infiltrated neutrophils produce NETs in the injured spinal cord. (A) Representative images of CitH3 (red) and MPO (green) double-positive cells in spinal cord from sham-operated rats and SCI rats at 24 h after operation. Nuclear was marked with DAPI (blue). Scale bars = 100 μm. (B) Representative images of network-like cell-free DNA structure by Sytox Orange staining. Scale bars = 200 μm. (C) Representative immunoblots and quantification of the CitH3 levels in spinal cord of rats subjected to SCI or sham operation. GAPDH is used as a loading control. Data are presented as means ± SD of n = 9 (*p < 0.05).