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. 2021 May 26;70(8):1717–1728. doi: 10.2337/db21-0037

Figure 2.

Figure 2

Static GSIS in β-cell CIC KO islets is indistinguishable from control islets. A and B: Islets were isolated from CICfl/fl; Ins1-Cre vs. CICfl/fl mice (10–20 weeks of age, n = 3–5 per genotype). Islets were incubated at basal (2.5 mmol/L) glucose followed by stimulation with glucose (12 mmol/L) alone, glucose and palmitate (0.5 mmol/L), or glucose and oleate/palmitate (1 mmol/L, 2:1) for 1 h each as indicated. C and D: Islets isolated from C57BL6/J mice (10–20 weeks of age, n = 3) were treated with triacsin C (10 μmol/L) for 72 h (C). Islets were sequentially incubated at basal (2.5 mmol/L) glucose followed by stimulatory (12 mmol/L) glucose for 1 h each as indicated (D). Insulin content was derived from whole-cell lysates. Data are mean ± SEM. *P < 0.05 by two-way ANOVA (A and B) or two-tailed unpaired t test (C and D). Glc, glucose.