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. 2021 May 26;70(8):1717–1728. doi: 10.2337/db21-0037

Figure 4.

Figure 4

Adult-inducible β-cell–specific deletion of CIC does not impair β-cell function. CICfl/fl; MIP-CreERT vs. CICfl/fl mice were analyzed 4–6 weeks after tamoxifen (i.p. injection). A: Isolated islets were analyzed for protein expression by immunoblot. B: CIC protein expression was normalized to tubulin and expressed as percent relative to CICfl/fl controls (n = 4). Four- to 6-h fasted male (C and D) (n = 16) and female (F and G) (n = 16) mice (12–16 weeks of age) were injected with glucose 1.5 mg/g body wt i.p. Blood glucose (C and F) and plasma insulin (D and G) were recorded at the indicated times. Isolated islets from male (E) (n = 8) and female (H) (n = 9) mice were perifused for 32 min at basal (2.5 mmol/L) glucose followed by stimulatory glucose (11.2 mmol/L). Data are mean ± SEM. *P < 0.05 by two-tailed unpaired t test (B) or repeated-measures two-way ANOVA (C–H). glc, glucose.