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. 2021 May 11;70(8):1738–1753. doi: 10.2337/db20-1268

Figure 7.

Figure 7

TRIB3 overexpression activates downstream signaling in Müller MIO-M1 cells. A: AGE treatment results in TRIB3 overexpression in 72 h (n = 6). B: Hypoxia induces HIF1 and EGFR protein in addition to TRIB3 (Fig. 1) (n = 4). C: Müller cells overexpressing TRIB3 demonstrate compromised cell viability (n = 4). D: Overexpression of TRIB3 in hypoxic Müller cells results in overexpression of EGFR1, HIF1α, VEGF, GFAP, and GLUT1 (n = 4–5). E: Knockdown of Hif1a mRNA in hypoxic Müller cells results in downregulation of GLUT1, EGFR1, and VEGF1, whereas GFAP expression does not respond to the treatment with siRNA (n = 5). Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. a.u., arbitrary units.