Fig. 5. 3E8 treatment had no effects on ACE2’s physiological functions.

a 3E8 treatment had no effects on enzymatic activities of recombinant human ACE2 protein. AF933, an ACE2 polyclonal goat antibody, was used as positive control. hIgG1-Fc and isotype were used as negative controls. b 3E8 treatment showed no effects on enzymatic activities of ACE2 molecules endogenously expressed on the surface of Vero E6 cells. c Total expression of ACE2 by Vero E6 cells was not affected by 3E8 treatment at 0.133, 0.667, or 6.67 nM for 24 h. d The levels of membrane ACE2 expression on HEK293 cells were reduced by 3E8 treatment but were stabilized after 24 h. *p < 0.05; **p < 0.01