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. 2021 Aug 11;12:719009. doi: 10.3389/fimmu.2021.719009

Figure 1.

Figure 1

HIF-1α is activated by hypoxia in MD-macrophages from high active sarcoidosis. (A, B) Representative immunofluorescence staining of MD-macrophages. (A) Similar presence of HIF-1α (in red) in controls and in high active pulmonary sarcoidosis (AS) and low active or inactive sarcoidosis (IS) patients in both normoxia and hypoxia. Pimonidazole staining (in green) of MD-macrophages from AS is absent in normoxia (magnification x200). (B) Nuclear staining with DAPI (in blue) and accumulation of HIF-1α (in red) in MD-macrophages. Overlay image showing HIF-1α nuclear localization (magnification x400) (same results were found in 3 patients and 3 controls). (C) HIF-1α activation (measured as OD 450nm) assessed by TransAM® HIF-1α in controls, AS and IS patients, after 24hrs of normoxia or hypoxia. Results are expressed as box plot showing 25th and 75th percentile and median, each point indicates a patient and/or control (n= 7/group). *p < 0.05; ****p < 0.0001 in two-way ANOVA-repeated measures with Sidak post-hoc test.