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. 2021 Aug 11;12:719009. doi: 10.3389/fimmu.2021.719009

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Copyright © 2021 Jeny, Bernaudin, Valeyre, Kambouchner, Pretolani, Nunes, Planès and Besnard

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hypoxia decreased CD80 and CD86 co-stimulation molecules and HLA-DR. (A–C) Effect of hypoxia on CD80, CD86 and HLA-DR surface expression on CD14+ MD-macrophages from controls, high active sarcoidosis (AS), and low active or inactive sarcoidosis (IS). Results are expressed as the mean fluorescence intensity (MFI) of CD80 (A), CD86 (B) and HLA-DR (C) analyzed by flow cytometry. Results are expressed as box plot showing 25^th and 75^th percentile and median, each point indicates a patient and/or control (n = 6-11/group). *p < 0.05; **p < 0.01; ****p < 0.0001 in two-way ANOVA-repeated measures with Sidak post-hoc test.