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. 2021 Aug 24;40:266. doi: 10.1186/s13046-021-02069-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Apatinib inhibited malignant growth and cancer stem cell-like properties in NSCLC cells. a Plate colony formation of A549 and H1299 cells after apatinib treatment for 14 days and quantitation of colony numbers. The data are presented as mean ± SD (n = 3). ^**p < 0.01 vs. control. b Soft agar colony formation of A549 and H1299 cells after apatinib treatment for 14 days and quantitation of colony numbers. Scar bar = 200 μm. The data are presented as mean ± SD (n = 3). ^**p < 0.01 vs. control. c Flow cytometry analysis of CD133-positive cells and quantification of the percentage of CD133-positive cells. The data are presented as mean ± SD (n = 3). ^**p < 0.01 vs. control