Fig. 4. Approaches to study site-specific O-GlcNAcylation in vivo. (a) Principle of a genetic code expansion approach with a metabolically stable Cys-S-GlcNAc amino acid: (1). UAA uptake, (2). tRNA aminoacylation, (3). UAA translation in response to a stop codon, (4). Production of a modified protein. (b) Genetic recoding approach to introduce site-specific S-GlcNAc mimicry of O-GlcNAcylation.24 Blue squares denote GlcNAc. POI – protein of interest.
