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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2020 Sep 23;408:115250. doi: 10.1016/j.taap.2020.115250

Figure 4. Hepatic lipid xenobiotic metabolism and transport gene expression.

Figure 4.

The quantification of selected liver mRNAs was performed using QuantiGene 2.0 Plex Assay kit with purified RNA. The fluorescence intensity (FI; minus background) for each gene was normalized to the housekeeping gene β-actin. Fold change was calculated between the control SD mice. A) PFOS treatment induced 7 out of 8 transcripts related to xenobiotic metabolism. B) HFD and PFOS treatment represses some transporter gene expression. Calculations were done using a one-way ANOVA followed by Fisher’s LSD test. All values are means ± SEM; N = 5. * indicates p<0.05 versus control SD, indicates p<0.05 for H-SD versus HFD, # indicates p<0.05 versus respective diet controls, † indicates p<0.05 for H-SD+PFOS versus SD+PFOS, and ҂ indicates p<0.05 for H-SD+PFOS versus HFD+PFOS.