Skip to main content
. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2020 Sep 23;408:115250. doi: 10.1016/j.taap.2020.115250

Table 4.

Effect of HFD on upstream regulators of the proteomic response

Upstream Regulator Name Function Activation Z-score Target Molecules in Data Set
Scap sterol regulatory element binding protein (SREBF) chaperone Unfolded protein response −2.200 Acly, Csad, Fasn, Fdps, Hmgcs2, Scd-1
Por cytochrome p450 oxidoreductase donate electrons directly from
NADPH to all microsomal P450 enzymes		 2.000 Acly, Bdh1, Cpt1a, Csad, Cyp2c8, Fdps, Scd-1

Protein data were analyzed using Perseus open source software. The numbers of significantly increased and decreased proteins for relevant comparisons were calculated (student’s t-test, p-value ≤ 0.05, false discovery rate (FDR) < 0.05). Diet based comparisons were SD/H-SD and SD/HFD. Differentially expressed proteins were further analyzed using Ingenuity Pathway Analysis (IPA)’s upstream analysis. Significant upstream regulatory molecules are described for SD vs HFD, and an activation z-score was calculated to predict activation of upstream regulators. A positive z-score indicates upregulation and a negative z-score indicates downregulation.