FIG. 1.
Introduction of synthetic telomeres into yeast. (A) Replacement of the left telomere of chromosome VII by homologous integration at the ADH4 locus, using a synthetic telomere adjacent to URA3. Different inserts, shown in panel B, were cloned between URA3 and the TG1-3 sequences to attempt to alter telomere length regulation. (B) The synthetic telomeres used in this work. The nontelomeric spacers between the TG1-3 tracts are described in Materials and Methods. The orientation of the TG1-3 repeats is indicated by arrowheads. YIpADH652-42 has the internal 256-bp TG1-3 tract in the reverse orientation relative to the terminal 29-bp tract. The specific YIpADHTEF and YIpADHλ telomeres are presented in Fig. 5. (C) Sequences of the TG1-3 repeats and spacer in the YIpADH256-50 construction. The sites used to construct the plasmids in panel B are indicated. The TG1-3 repeats are from positions 54 to 309 in the 5′ KpnI site, and the single A in the TG1-3 repeats is at position 98.