TABLE 1.
Proteins identified from immunoprecipitation by mass spectrometrya
| Protein name | GenPept accession no. | No. of peptides (no. unique) in: |
Mass (kDa) | |
|---|---|---|---|---|
| PE_PGRS20 | PE_PGRS47 | |||
| PE_PGRS20 | ||||
| Protein flightless-1 homolog | Q9JJ28 | 7 (7) | 144.7 | |
| Dedicator of cytokinesis protein 2 | Q8C3J5 | 4 (4) | 211.6 | |
| Myb-binding protein 1A | Q7TPV4 | 2 (2) | 151.9 | |
| Trifunctional enzyme subunit alpha. Mitochondrial | Q8BMS1 | 2 (2) | 82.6 | |
| PE_PGRS47 | ||||
| Lysozyme C-1 | P17897 | 2 (1) | 16.8 | |
| PE_PGRS20 and PE_PGRS47 | ||||
| Ras GTPase-activating-like protein IQGAP1 | Q9JKF1 | 13 (13) | 5 (5) | 188.6 |
| MICOS complex subunit Mic19 | Q9CRB9 | 6 (6) | 3 (3) | 26.3 |
| Endoplasmic reticulum chaperone BiP | P20029 | 4 (3) | 10 (9) | 72.4 |
| Erythrocyte band 7 integral membrane protein | P54116 | 4 (4) | 5 (5) | 31.4 |
| 14-3-3 protein gamma | P61982 | 4 (3) | 3 (3) | 28.3 |
| Endoplasmin | P08113 | 4 (4) | 5 (5) | 92.4 |
| ATP synthase subunit beta, mitochondrial | P56480 | 3 (3) | 3 (3) | 56.3 |
| Sodium/potassium-transporting ATPase subunit alpha-1 | Q8VDN2 | 3 (3) | 2 (2) | 112.9 |
| Galectin-3 | P16110 | 2 (2) | 2 (2) | 27.5 |
| Phosphate carrier protein, mitochondrial | Q8VEM8 | 2 (2) | 2 (2) | 39.6 |
| Ras-related protein Rab-1A | P62821 | 2 (2) | 2 (2) | 22.7 |
a
Proteins were identified in duplicate samples from two independent experiments. Proteins identified in empty vector control and nonspecific proteins commonly identified by these methods (33) were removed.