FIG 6.
PIK-75 is a cell wall active compound that induces cell cycle defects. (A) MICs of PIK-75 against C. neoformans H99 at 30°C versus 37°C and YPD ± 1 M sorbitol. (B) Spot dilutions of H99 on YPD containing 1 μg/ml PIK-75 with 0.025% SDS. Plates were incubated at 37°C for 48 h. (C) Phosphorylated Mpk1 Western blot of cells treated with DMSO or 4 μg/ml PIK-75, and then challenged with CFW for 0, 1, or 2 h. Ponceau staining is shown as loading control. (D) Brightfield and CFW staining of H99 treated with 2 μg/ml PIK-75 for 24 h at 37C. (E) Merged image of brightfield and GFP channels of H99Nop1-GFP cultured under the same conditions as in panel C. All images were taken using the same exposure and are representative of at least three independent experiments.