FIG 4.

Significantly higher midgut infection and dissemination for DENV-4H than for DENV-4L point to a potential midgut barrier for the laboratory virus strain. Data shown are for Aedes aegypti (ORL) 7 and 10 days after infectious blood feeding with the DENV-4 Haiti (H) (red) or the DENV-4 laboratory (L) (blue) strain. (A) Midgut and body infection rates; (B) day 7 midgut and body infection intensities; (C) day 10 midgut and body infection intensities. Dots represent individual mosquito samples, with medians and 95% confidence intervals per group combined from three biological replicates. *, P value of <0.05 via Fisher’s exact test for infection rates and Kruskal-Wallis and Dunn’s post hoc tests for infection intensity.