TABLE 1.
Plasmids and primers used in this study
Plasmid or primer | Characteristic or sequence | Source |
---|---|---|
pMTL0007C-E2: ArcB-156a | ClosTron plasmid targeted to the Hall A-hyper arcB gene (CLC2465) | DNA2.0 (Atum) |
pMTL0007C-E2: ArgG-147a | ClosTron plasmid targeted to the Hall A-hyper argG gene (CLC2544) | DNA2.0 (Atum) |
ArcB-32F | CACTAATGGATTTCACACCAAAGGA | Integrated DNA Technologies |
ArcB-507R | TGCCATATTATTAGCACCATCTCCA | |
ArgG-18F | GCTTGCATACTCAGGAGGATTAGAT | Integrated DNA Technologies |
ArgG-409R | TAGGATCTTGAGCCTTTACTCCAAC | |
A1-500F | GCTTTGGACATGAAGTTTTGAATC | Integrated DNA Technologies |
A1-926R (426 bp) | GAAGCAGTAGTACCTACTATTGATTTAGC | |
A1-1026F | CAAAATGTTAACAGAGATTTACACAGAG | Integrated DNA Technologies |
A1-1825R (799 bp) | GTTCTACCCAGCCTAAAAACATAG | |
A1-1990F | CTGTTAGAATTTATACCAGAGATTGC | Integrated DNA Technologies |
A1-2701R (711 bp) | CTATTGGATCAAAATTTACTTTACTACC |