TABLE 3.
BUSCO analysis of Naegleria species annotated protein sequencesa
| BUSCO type | No. of BUSCOs in: |
|||||
|---|---|---|---|---|---|---|
| N. fowleri (TY)b | N. fowleri (ATCC 30894)c | N. fowleri (ATCC 30863)c | N. lovaniensis (ATCC 30569)c | N. lovaniensis (76-15-250) | N. gruberi (ATCC 30224)c | |
| Complete | 220 | 219 | 210 | 220 | 220 | 203 |
| Duplicated | 3 | 2 | 7 | 16 | 4 | 1 |
| Fragmented | 7 | 7 | 8 | 10 | 9 | 14 |
| Missing | 28 | 29 | 37 | 25 | 26 | 38 |
a
Genome completeness was evaluated by analyzing 255 conserved BUSCOs (v4.0.6) of the Eukaryota odb10 data set. Publicly available protein coding sequences were downloaded from figshare (https://doi.org/10.6084/m9.figshare.8313656) for N. fowleri (ATCC 30894), while for the rest of the published genomes, the reannotated protein sequences using Braker2 were used for the BUSCO analysis.
b
Out of 52 N. fowleri genomes sequenced, BUSCO analysis is shown only for the “close-to-complete” genome of the N. fowleri TY isolate.
c
Published Naegleria genomes.