FIG 4.

Concentration (pg/ml) of the investigated cytokines released into the supernatant of the 3D granuloma structure and traditional cell culture control (monolayer) extracellular environments, as measured by Luminex analysis. The cytokines measured included (a) IL-10, (b) TNF-α, (c) IFN-γ, (d) IL-2, and (e) IL-22. Cytokine production was compared between initial production by uninfected and that by BCG-infected AM 2 days post culture initiation and subsequent release until the end of culture (5 days post culture initiation), as well as compared to the corresponding cytokine release by the BCG-infected chronic traditional cell culture control (monolayer) and autologous CD3⁺ T cells prior to addition to the AM culture. Each data point represents the median of three individual participants, with BCG infection occurring on day 1 after culture initiation (the day of culture initiation is considered day 0). The day 2 inset for each cytokine depicts the differences between cytokines released by uninfected and BCG-infected AM 2 days post culture initiation, i.e., 1 day postinfection (each data point represents a single individual from the three individual participants assessed).