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Size exclusion chromatograms (left) and accompanying SDS-PAGE gels (right) assessing complex formation between RH5/RBD/FL-S/RBD and ACE2/basigin. Symbols on chromatograms indicate which gels correspond to the peaks. Full-length RH5 (∼60 kDa) undergoes cleavage at room temperature to yield an ∼43-kDa band. (A) RBD-ACE2; (B) FL-S-ACE2; (C) RH5-ACE2; (D) RBD-basigin; (E) FL-S–basigin; (F) RH5-basigin; (G) FL-NP–basigin.
