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. 2021 Jul 21;6(4):e00318-21. doi: 10.1128/mSphere.00318-21

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Copyright © 2021 Crisan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Vibrio cholerae strain BGT49 encodes the Aux 4 T6SS cluster and efficiently eliminates target bacteria in a TpeV- and T6SS-dependent manner. (A) The Aux 4 cluster encodes predicted hcp, vgrG, DUF4123-containing chaperone, effector, immunity, and paar genes. The cluster is found on a predicted mobile genetic element, flanked by integrase and transposase genes. (B) Target V. cholerae BGT49 ΔtpeV ΔtpiV (CC170) was cocultured with either WT, ΔtpeV (CC167), or ΔvasK T6SS⁻ (CC168) BGT49. A one-way analysis of variance (ANOVA) with a post hoc Tukey honestly significant difference (HSD) test was used to determine significance. ****, P < 0.0001.