FIG 2.

V. cholerae C6706* can use the Aux 4 cluster to eliminate target cells in a T6SS-dependent manner. (A) V. cholerae C6706* (T6SS⁺ or T6SS⁻) with a plasmid control or a plasmid encoding the Aux 4 cluster was cocultured with target parental V. cholerae C6706. A one-way ANOVA with a post hoc Tukey HSD test was used to determine significance. (B) Killer V. cholerae C6706* with the Aux 4 cluster was cocultured with target C6706 cells with a plasmid control or a plasmid encoding tpiV. Welch’s t test was used to determine significance. (C) V. cholerae C6706* with deletions in the known tseL and vasX T6SS effectors containing either a plasmid control or a plasmid with Aux 4 was cocultured with E. coli MG1655 cells. A one-way ANOVA with a post hoc Tukey HSD test was used to determine significance. (D) V. cholerae C6706* with a plasmid control or a plasmid encoding the Aux 4 cluster was cocultured with E. coli MG1655 on LB medium with 0.4% glucose. A one-way ANOVA with a post hoc Tukey HSD test was used to determine significance. ****, P < 0.0001; ***, P < 0.001; **, P < 0.01.