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. 2021 Aug 11;9:722412. doi: 10.3389/fcell.2021.722412

FIGURE 3.

FIGURE 3

Cysteine, but not NaHS, is able to rescue the ATP synthesis impairment, after 48 h under hypoxia triggered by xCT inhibition. (A–C) ATP levels for 1 h of experimental conditions for (C). ES2 and OVCAR3 cells under hypoxia with and without cysteine and in the presence of the H2S donor, NaHS sulfasalazine in which n = 5 for ES2; and H sulfasalazine and H NaHS C sulfasalazine, in which n = 5 for OVCAR3. (D,E) ATP levels for 48 h of experimental (un-normalized data), and (D) ES2 and (E) OVCAR3 cells under hypoxia with and without cysteine supplementation and in the presence of the xCT inhibitor, conditions for (A) ES2 and (B) OVCAR3 cells under hypoxia with and without cysteine and in the presence of the xCT inhibitor, sulfasalazine and the H2S donor, NaHS. Data were normalized to the respective control condition (the same environmental condition H/HC without NaHS or sulfasalazine). Data are presented as log2 fold change (n = 6 for both cell lines). H, hypoxia; HC, hypoxia with cysteine. Results are shown as mean ± SD. */p < 0.05, **/##p < 0.01, and ***/###p < 0.001 [one-way ANOVA with post hoc Tukey tests for (A,B,D,E) and independent-samples t-test for (C)].