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. 2021 Aug 11;9:722412. doi: 10.3389/fcell.2021.722412

FIGURE 4.

FIGURE 4

Cysteine rescues ES2 cellular metabolism and impacts several metabolic pathways under hypoxia. (A–F) Metabolites levels for 48 h of experimental conditions for ES2 cells. (A) Intracellular amino acids; (B) intracellular sugars and organic acids; (C) other intracellular metabolites; (D) amino acids in supernatants; (E) sugars and organic acids in supernatants; (F) other metabolites in supernatants. Data were normalized to the respective control condition (the same environmental condition NC/N and HC/H). NC, normoxia with cysteine; HC, hypoxia with cysteine. Results are shown as median with 25th to 75th percentiles. *p < 0.05, **p < 0.01, ***p < 0.001 (independent-samples t-test: NC, n = 6; HC, n = 5). (G) Metabolic pathway analysis for the effect of cysteine under normoxia and hypoxia in intracellular ES2 metabolites. All the matched pathways are displayed as circles. The color and size of each circle are based on p-value and pathway impact value, respectively. The most impacted pathways having statistical significance (p < 0.05) are indicated. Source: https://www.metaboanalyst.ca/Metabo Analyst/faces/home.xhtml.