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. 2021 Aug 11;9:722412. doi: 10.3389/fcell.2021.722412

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Copyright © 2021 Nunes, Ramos, Santos, Mendes, Silva, Vicente, Pereira, Félix, Gonçalves and Serpa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of cysteine in OVCAR3 metabolites under normoxia and hypoxia. Metabolites levels for 48 h of experimental conditions for OVCAR3 cells. (A) Intracellular amino acids; (B) intracellular sugars and organic acids; (C) other intracellular metabolites; (D) amino acids in supernatants; (E) sugars and organic acids in supernatants; (F) other metabolites in supernatants. Data were normalized to the respective control condition (the same environmental condition NC/N and HC/H). NC, normoxia with cysteine; HC, hypoxia with cysteine. Results are shown as median with 25th to 75th percentiles. *p < 0.05, **p < 0.01, ***p < 0.001 (independent-samples t-test: NC, n = 6; HC, n = 6).