Table 2.
Oligonucleotides used in this study.
| Oligonucleotide | Description | Sequence |
|---|---|---|
| Rmnd5a (mm) fwd | genotyping PCR | TTGTTCTGACACAGTGCTGC |
| Rmnd5a (mm) rev | genotyping PCR | ACACGAGGCTTCCCATCAAT |
| Hprt (mm) fwd | qPCR | TACAGGCCAGACTTTGTTGG |
| Hprt (mm) rev | qPCR | AACTTGCGCTCATCTTAGGC |
| Mkln1 (mm) fwd | qPCR | TGTGGATCATTCGGACCCAG |
| Mkln1 (mm) rev | qPCR | TCTGAAAAGCCTAGAGCTGTGA |
| Rmnd5a (mm) fwd #1 | qPCR | CAGCCAACGGCTTCTCAATG |
| Rmnd5a (mm) rev #1 | qPCR | GACAGACCAGATTCCTGGCA |
| Rmnd5a (mm) fwd #2 | qPCR | GCCTGTCCCATTCTTCGTCA |
| Rmnd5a (mm) rev #2 | qPCR | GGACTCTGTTCCATTGGGCA |
| Rmnd5b (mm) fwd | qPCR | GTGGGCCAGCTGAGAGC |
| Rmnd5b (mm) rev | qPCR | AGCACTGGGACATCACAAGG |
| siMkln1 (mm) #1 | siRNA | CACUUCAGACAACAUAACU |
| siMkln1 (mm) #2 | siRNA | AGUUAUGUUGUCUGAAGUG |
| siRmnd5a (mm) #1 | siRNA | CAGGCUGAUGUGAGAAUGAAA |
| siRmnd5a (mm) #2 | siRNA | UUGGUUUGUGGUCAUAUUAUA |
| siIft88 (mm) #1 | siRNA | ACUGGGAGAGUUAUACGAU |
| siIft88 (mm) #2 | siRNA | AUCGUAUAACUCUCCCAGU |
| sgRmnd5a (mm) seq #1 | sgRNA | CATAGCAGTGTTTCTCGAGT |
| sgRmnd5a (mm) seq #2 | sgRNA | ACAAGGAATGCTGGATGTAG |
| sgRmnd5a (mm) seq #3 | sgRNA | CATAGCCCAAAACAGTTCCT |
| Prkaa1 (mm) fwd | qPCR | GTCAAAGCCGACCCAATGATA |
| Prkaa1 (mm) rev | qPCR | CGTACACGCAAATAATAGGGGTT |
| Prkaa2 (mm) fwd | qPCR | AAGATCGGACACTACGTCCTG |
| Prkaa2 (mm) rev | qPCR | TGCCACTTTATGGCCTGTCAA |
| tba1 (ce) fwd | qPCR | TCAACACTGCCATCGCCGCC |
| tba1 (ce) rev | qPCR | TCCAAGCGAGACCAGGCTTCAG |
| gid1/y54e5a.7 (ce) fwd | qPCR | ACGTAACCTATTCACCGGTTGG |
| gid1/y54e5a.7 (ce) rev | qPCR | CTCGAGAATCATTTTCCGGACG |
| gid2/t07d1.2 (ce) fwd | qPCR | TGACGAGCAAGGAAGTAGCTG |
| gid2/t07d1.2 (ce) rev | qPCR | CAAGCCCGAATTGCGTTGAC |
| gid7/y39h10a.6 (ce) fwd | qPCR | TTCGTTCATCGCAGTGGACA |
| gid7/y39h10a.6 (ce) rev | qPCR | CGGTTTCTTTTCCAATCGAGCC |
| gid8/f53e2.1 (ce) fwd | qPCR | ATCCGAACGAGAACAGACGG |
| gid8/f53e2.1 (ce) rev | qPCR | GCGAACATCCCGTAAAAGCG |
| AMPKT183A | Mutagenesis | CAGATGGTGAATTTTTAAGAGCAAGCTGTGGCTCACCCAATTATG |