Figure 3.

Effect of low oxygen tension (1% O₂) on the expression of TFEB protein and mRNA, lysosomal proteome, and TFEB nuclear translocation in primary human trophoblasts. Primary trophoblasts from three different preparations were cultured under normoxic or hypoxic (1% O₂) conditions. (A–C) TFEB protein abundance and mRNA levels were significantly decreased in hypoxia-treated cells. (D, E) LAMP1, LAMP2, and CTSD were significantly reduced in primary trophoblasts in response to hypoxia. (F) TFEB immunofluorescent signals were predominantly concentrated in the nuclei (arrows) of the cells under normoxic, not hypoxic, condition. Cells were cultured in media containing 2.5% FBS. Images are representatives of 3 independent experiments. Bar: 10 μm. Data are presented as mean ± SEM and analyzed by a Student t-test (n = 3).