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. 2019 Dec 26;16(9):1635–1650. doi: 10.1080/15548627.2019.1703353

Figure 1.

Figure 1.

c9orf72 and smcr8 dKO mice exhibit more severe splenomegaly and lymphadenopathy than individual KOs. (A) Gross images of cervical lymphadenopathy (arrow) in 7-month-old smcr8−/- mice compared to wild type littermate controls. White arrows indicate the enlarged lymph nodes in mutant mice. Scale bar: 0.5 cm. (B and D) Gross images of lymph nodes (B) and spleens (D) dissected from 7-month-old mice. Scale bar = 5 mm. (C and E) Statistical analysis of lymph node and spleen weights. Error bars represent SEM of 3 independent experiments; *p < 0.05, ***p < 0.001 (Student’s t test). Two-way ANOVA detected a significant difference between 2.5 and 7 months of age. (F-H) Western blot analysis of C9orf72 and SMCR8 protein expression in macrophages derived from mutant mice. (I and K) Gross images of lymph nodes (I) and spleens (K) dissected from 3-month-old mice. Scale bars: 1.5 mm (I), and 1 mm (K). (J and L) Statistical analysis of lymph node and spleen weights. Error bars represent SEM of 3 independent experiments followed by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01). n.s represents not significant.